%0 Journal Article
%T Preparation of Three
%A Alanna Burwell
%A Barbara F. Williams
%A Darlene Dixon
%A Heather Jensen
%A Natasha P. Clayton
%A Pamela Ovwigho
%A Quashana D. Brown
%A Sreenivasa Ramaiahgari
%A Tonia Hermon
%J Toxicologic Pathology
%@ 1533-1601
%D 2018
%R 10.1177/0192623318789069
%X The use of three-dimensional (3-D) in vitro culture systems (spheroids, organoids) in biomolecular and drug discovery research has become increasingly popular. The popularity is due, in part, to a diminished reliance on animal bioassays and a desire to develop physiologically relevant cell culture systems that simulate the in vivo tissue microenvironment. Most evaluations of 3-D cultures are by confocal microscopy and high-content imaging; however, these technologies do not allow for detailed cellular morphologic assessments or permit basic hematoxylin and eosin histologic evaluations. There are few studies that have reported detailed processes for preparing 3-D cultures for paraffin embedding and subsequent use for histochemical or immunohistochemical staining. In an attempt to do so, we have developed a protocol to paraffin-embed human liver spheroids that can be sectioned with a microtome and mounted onto glass slides for routine histochemical and immunohistochemical staining and light microscopic evaluations
%K 3-D cultures
%K HepaRG spheroids
%K in vitro
%K histochemistry
%K immunohistochemistry
%K light microscopy
%K liver spheroids
%U https://journals.sagepub.com/doi/full/10.1177/0192623318789069