%0 Journal Article
%T Utility of G protein
%A Anne Israelsson
%A Basel Sitohy
%A Gudrun Lindmark
%A Haytham Ali
%A Lina Olsson
%A Manar AbdelMageed
%A Marie-Louise Hammarstr£¿m
%A Sten Hammarstr£¿m
%J Tumor Biology
%@ 1423-0380
%D 2019
%R 10.1177/1010428319858885
%X The utility of mRNA and protein determinations of G protein-coupled receptor 35, that is, GPR35a (GPR35 V1) and GPR35b (GPR35 V2/3), as indicators of outcome for colon cancer patients after curative surgery was investigated. Expression levels of V1 and V2/3 GPR35, carcinoembryonic antigen and CXCL17 mRNAs were assessed in primary tumours and regional lymph nodes of 121 colon cancer patients (stage I¨CIV), colon cancer cell lines and control colon epithelial cells using real-time quantitative reverse transcriptase-polymerase chain reaction. Expression of G protein-coupled receptor 35 was investigated by two-colour immunohistochemistry and immunomorphometry. GPR35 V2/3 mRNA, but not V1 mRNA, was expressed in colon cancer cell lines, primary colon tumours and control colon epithelial cells. Haematoxylin and eosin positive (H&E(+)), but not H&E(¨C), lymph nodes expressed high levels of GPR35 V2/3 mRNA (P<0.0001). GPR35b and carcinoembryonic antigen proteins were simultaneously expressed in many colon cancer tumour cells. Kaplan¨CMeier and hazard ratio analysis revealed that patients with lymph nodes expressing high levels of GPR35 V2/3 mRNA and, in particular, in the group of patients with lymph nodes also expressing carcinoembryonic antigen mRNA, had a short disease-free survival time, 67£¿months versus 122£¿months at 12-year follow-up (difference: 55£¿months, P£¿=£¿0.001; hazard ratio: 3.6, P£¿=£¿0.002). In conclusion, high level expression of G protein-coupled receptor 35 V2/3 mRNA in regional lymph nodes of colon cancer patients is a sign of poor prognosis
%K GPR35b
%K GPR35 V1 mRNA
%K GPR35 V2/3 mRNA
%K CXCL17 mRNA
%K CEA
%K colon cancer
%K qRT-PCR
%K immunohistochemistry
%U https://journals.sagepub.com/doi/full/10.1177/1010428319858885