%0 Journal Article
%T Portable Chronic Alcohol Consumption Monitor in Human Sweat through Square
%A Anjan Panneer Selvam
%A David Kinnamon
%A Shalini Prasad
%A Sriram Muthukumar
%J SLAS TECHNOLOGY: Translating Life Sciences Innovation
%@ 2472-6311
%D 2018
%R 10.1177/2472630317733255
%X Chronic alcohol consumption is a significant financial and physical burden in the United States each year. Alcohol consumption monitors focus on establishing a state of intoxication, not assessing a user¡¯s health risks as a function of consumed alcohol. This work demonstrates a biosensor for a chronic alcohol consumption monitor through the electrochemical detection of ethyl glucuronide (EtG) in human sweat using square-wave voltammetry (SWV). A novel affinity assay was demonstrated in which monoclonal antibodies were chemically coabsorbed onto a gold electrode surface in parallel with thiolated charge transfer molecule. Concentration-dependent EtG binding was detected by measuring a reduction in the charge transfer of the sensor, manifesting as a current response during SWV measurement. A companion compact electronic reader was constructed, demonstrating comparable sensitivity to a conventional lab instrument. Both tools demonstrated a limit of detection of 0.1 ¦Ìg/L and a linear dynamic range of 0.1¨C100 ¦Ìg/L corresponding to the physiologically relevant range of EtG expression in human sweat. This device can address the need for a chronic alcohol consumption monitor toward establishing a user¡¯s long-term consumption habits to assess the risk of developing specific diseases and conditions associated with regular alcohol consumption, through integration with existing technologies
%K electrochemical impedance spectroscopy (EIS)
%K square-wave voltammetry (SWV)
%K ethyl glucuronide (EtG)
%K portable electronics
%K affinity assay
%U https://journals.sagepub.com/doi/full/10.1177/2472630317733255