%0 Journal Article
%T Determination of Mercuric Ion in Water Samples with a LED Exciting and CCD Based Portable Spectrofluorimeter
%A Arsenio Mu£¿oz de la Pe£¿a
%A Mar¨ªa Isabel Rodr¨ªguez-C¨¢ceres
%A Diego Bohoyo Gil
%A Mar¨ªa del Carmen Mahedero
%A Mar¨ªa del Carmen Hurtado-S¨¢nchez
%A Reyes Babiano
%J American Journal of Analytical Chemistry
%P 605-611
%@ 2156-8278
%D 2011
%I Scientific Research Publishing
%R 10.4236/ajac.2011.25068
%X The fluorescent characteristics of a fluorimetric chemosensor for mercuric ion, Hg2+, employing a synthesized Rhodamine 6G derivative, have been analyzed. For that, a portable spectrofluorimeter composed of a 515 nm LED as excitation source, two fiber-optics and a CCD camera as detector, has been used, intended for ¡°in situ¡± analysis. A highly selective Rhodamine based probe for Hg2+, that is water soluble and gives a positive response upon analyte binding, is reported. The reagent is bearing a monothiospirolactone group in a Rhodamine 6G architecture and the thiol atom served for the direct attack of thiophilic Hg2+. The fluorescence enhancement is attributed to the spirolactone ring opening and the coordination of two sulphur atoms to Hg2+ giving a 2:1 reagent: Hg2+ stoichiometry complex.
%K Chemosensor
%K LED
%K Mercuric Ion
%K Water Analysis
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=7301