%0 Journal Article
%T Continuous and Quantitative Purification of T
%A Andrew Jann
%A Coleman Murray
%A Da Eun Park
%A Dino Di Carlo
%A Edward Pao
%J SLAS TECHNOLOGY: Translating Life Sciences Innovation
%@ 2472-6311
%D 2018
%R 10.1177/2472630317748655
%X T-cell-based immunotherapies represent a growing medical paradigm that has the potential to revolutionize contemporary cancer treatments. However, manufacturing bottlenecks related to the enrichment of therapeutically optimal T-cell subpopulations from leukopak samples impede scale-up and scale-out efforts. This is mainly attributed to the challenges that current cell purification platforms face in balancing the quantitative sorting capacity needed to isolate specific T-cell subsets with the scalability to meet manufacturing throughputs. In this work, we report a continuous-flow, quantitative cell enrichment platform based on a technique known as ratcheting cytometry that can perform complex, multicomponent purification targeting various subpopulations of magnetically labeled T cells directly from apheresis or peripheral blood mononuclear cell (PBMC) samples. The integrated ratcheting cytometry instrument and cartridge demonstrated enrichment of T cells directly from concentrated apheresis samples with a 97% purity and an 85% recovery of magnetically tagged cells. Magnetic sorting of different T-cell subpopulations was also accomplished on chip by multiplexing cell surface targets onto particles with differing magnetic strengths. We believe that ratcheting cytometry¡¯s quantitative capacity and throughput scalability represents an excellent technology candidate to alleviate cell therapy manufacturing bottlenecks
%K ratcheting cytometry
%K cell therapy manufacturing
%K immunotherapy
%U https://journals.sagepub.com/doi/full/10.1177/2472630317748655