%0 Journal Article
%T Adipose
%A Charles F Reese
%A Elena Tourkina
%A Gustavo Carmen-Lopez
%A Kristi Helke
%A Marina Zemskova
%A Martin Introna
%A Michael Bonner
%A Nicoletta Del Papa
%A Rebecca Lee
%A Stanley Hoffman
%J Journal of Scleroderma and Related Disorders
%@ 2397-1991
%D 2019
%R 10.1177/2397198318821510
%X The potential value of mesenchymal stromal/stem cell therapy in treating skin fibrosis in scleroderma (systemic sclerosis) and of the caveolin-1 scaffolding domain peptide in treating lung, skin, and heart fibrosis is known. To understand how these observations may relate to differences between mesenchymal stromal/stem cells from healthy subjects and subjects with fibrosis, we have characterized the fibrogenic and adipogenic potential of adipose-derived mesenchymal stromal/stem cells from systemic sclerosis patients, from mice with fibrotic lung and skin disease induced by systemic bleomycin treatment, and from healthy controls. Early passage systemic sclerosis adipose-derived mesenchymal stromal/stem cells have a profibrotic/anti-adipogenic phenotype compared to healthy adipose-derived mesenchymal stromal/stem cells (low caveolin-1, high ¦Á-smooth muscle actin, high HSP47, low pAKT, low capacity for adipogenic differentiation). This phenotype is mimicked by treating healthy adipose-derived mesenchymal stromal/stem cells with transforming growth factor beta or caveolin-1 small interfering RNA and is reversed in systemic sclerosis adipose-derived mesenchymal stromal/stem cells by treatment with caveolin-1 scaffolding domain peptide, but not scrambled caveolin-1 scaffolding domain peptide. Similar results were obtained with adipose-derived mesenchymal stromal/stem cells from systemic sclerosis patients and from bleomycin-treated mice, indicating the central role of caveolin-1 in mesenchymal stromal/stem cell differentiation in fibrotic disease
%K Caveolin-1
%K fibrosis
%K adipogenesis
%K mesenchymal stem cells
%K scleroderma
%U https://journals.sagepub.com/doi/full/10.1177/2397198318821510