%0 Journal Article
%T Trophoblast
%A Cong-Jian Xu
%A Deng-Xuan Fan
%A Li-Ping Jin
%A Ming-Qing Li
%A Wen-Jie Zhou
%A Xiang-Hong Xu
%J Reproductive Sciences
%@ 1933-7205
%D 2019
%R 10.1177/1933719118777638
%X Decidual ¦Ã¦Ä T cells are known to regulate the function of trophoblasts at the maternal¨Cfetal interface; however, little is known about the molecular mechanisms of cross talk between trophoblast cells and decidual ¦Ã¦Ä T cells. Expression of chemokine C-X-C motif ligand 6 (CXCL16) and its receptor CXCR6 was evaluated in first-trimester human villus and decidual tissues by immunohistochemistry. ¦Ã¦Ä T cells were isolated from first-trimester human deciduae and cocultured with JEG3 trophoblast cells. Cell proliferation and apoptosis-related molecules, together with cytotoxicity factor and cytokine production, were measured by flow cytometry analysis. Expression of CXCL16 and CXCR6 was reduced at the maternal¨Cfetal interface in patients who experienced unexplained recurrent spontaneous abortion as compared to healthy pregnancy women. With the administration of pregnancy-related hormones or coculture with JEG3 cells, CXCR6 expression was upregulated on decidual ¦Ã¦Ä T cells. CXCL16 derived from JEG3 cells caused a decrease in granzyme B production of decidual ¦Ã¦Ä T cells. In addition, decidual ¦Ã¦Ä T cells educated by JEG3-derived CXCL16 upregulated the expression of Bcl-xL in JEG3 cells. This study suggested that the CXCL16/CXCR6 axis may contribute to maintaining normal pregnancy by reducing the secretion of cytotoxic factor granzyme B of decidual ¦Ã¦Ä T cells and promoting the expression of antiapoptotic marker Bcl-xL of trophoblasts
%K decidual ¦Ã¦Ä T cells
%K trophoblasts
%K CXCL16
%K CXCR6
%K unexplained recurrent spontaneous abortion
%U https://journals.sagepub.com/doi/full/10.1177/1933719118777638