%0 Journal Article
%T Rho
%A Akito Kakiuchi
%A Kenichi Takano
%A Shin Kikuchi
%A Takafumi Ninomiya
%A Takashi Kojima
%A Takayuki Kohno
%A Takumi Konno
%A Takuya Kakuki
%A Tetsuo Himi
%A Tomohiro Hata
%A Yakuto Kaneko
%A Yukino Hosaka
%J Journal of Histochemistry & Cytochemistry
%@ 1551-5044
%D 2019
%R 10.1369/0022155419841013
%X Primary cilia, regulated via distinct signal transduction pathways, play crucial roles in various cellular behaviors. However, the full regulatory mechanism involved in primary cilia development during cellular differentiation is not fully understood, particularly for the sensory hair cells of the mammalian cochlea. In this study, we investigated the effects of the Rho-kinase inhibitor Y27632 and PKC¦Į inhibitor GF109203X on primary cilia-related cell behavior in undifferentiated and differentiated temperature-sensitive mouse cochlear precursor hair cells (the conditionally immortalized US/VOT-E36 cell line). Our results indicate that treatment with Y27632 or GF109203X induced primary cilia elongation and tubulin acetylation in both differentiated and undifferentiated cells. Concomitant with cilia elongation, Y27632 treatment also increased Hook2 and cyclinD1 expression, while only Hook2 expression was increased after treatment with GF109203X. In the undifferentiated cells, we observed an increase in the number of S and G2/M stage cells and a decrease of G1 cells after treatment with Y27632, while the opposite was observed after treatment with GF109203X. Finally, while both treatments decreased oxidative stress, only treatment with Y27632, not GF109203X, induced cell cycle-dependent cell proliferation and cell migration
%K cell cycle
%K cell migration
%K Hook2
%K mouse cochlear cells
%K oxidative stress
%K PKC¦Į inhibitor GF109203X
%K primary cilia
%K Rho-kinase inhibitor Y27632
%U https://journals.sagepub.com/doi/full/10.1369/0022155419841013