%0 Journal Article
%T MMP
%A C. Huang
%A F.R. Tay
%A H. Sun
%A H. Yang
%A J. Guo
%A S. Hong
%A W. Lei
%A Y. Tang
%J Journal of Dental Research
%@ 1544-0591
%D 2019
%R 10.1177/0022034519831931
%X Currently available drug delivery systems for oral diseases suffer from short retention time and poor local concentrations at the target site. A biodegradable stimulus-responsive hydrogel was synthesized in the present study to evaluate its application as an environmentally sensitive carrier for on-demand intraoral drug delivery. The hydrogel was synthesized from diacrylate-containing polyethylene glycol¨Cbased scaffolds and a cysteine-terminated peptide crosslinker (CGPQG¡ýIWGQC) via a Michael-type addition reaction. Because CGPQG¡ýIWGQC can be cleaved by matrix metalloproteinase 8 (MMP-8), minocycline hydrochloride, bovine serum albumin, or an antibacterial peptide (KSL) was incorporated into the scaffolds to evaluate the MMP-8-responsive release behavior of the on-demand drug delivery system. Hydrogel characterization and gelation kinetics were examined with gel time, Fourier-transform infrared spectroscopy, scanning electron microscopy, and measurements of rheologic parameters. Degradation behavior and MMP-8-responsive drug release were performed by high-performance liquid chromatography and protein-specific assay. Biocompatibility evaluation indicated that the hydrogels were noncytotoxic. Antibacterial testing demonstrated that the released drugs were able to maintain bioactivity. Taken together, these results suggest that the MMP-8-sensitive hydrogel is a promising candidate for on-demand intraoral localized drug delivery. Because MMP-8 is one of the most important biomarkers for periodontitis, the MMP-8-responsive hydrogel has potential to be used for in situ adaptive degradation in response to chronic periodontitis and peri-implantitis. This notion has to be tested in animal models of periodontal disease
%K drug delivery systems
%K controlled release
%K peptides
%K matrix metalloproteinases
%K periodontitis
%K peri-implantitis
%U https://journals.sagepub.com/doi/full/10.1177/0022034519831931