%0 Journal Article
%T Overexpression of Collapsin Response Mediator Protein 1 Inhibits Human Trophoblast Cells Proliferation, Migration, and Invasion
%A Chong Qiao
%A Chunhui Wang
%A Lei Zhu
%A Ling Huang
%A Man Sun
%A Na Li
%A Ruijing Chang
%A Ruochen Wang
%A Yuanyuan Li
%A Yue Hou
%J Reproductive Sciences
%@ 1933-7205
%D 2019
%R 10.1177/1933719118799214
%X Collapsin response mediator protein 1 (CRMP-1) is widely expressed in the nervous system and has tumor suppressive effects. Our previous studies have demonstrated that CRMP-1 was expressed in the trophoblasts of the whole stage of pregnancy with significantly increasing expression in the placenta of early-onset preeclampsia. Preeclampsia, especially early onset, is strongly associated with the dysfunction of trophoblast including proliferation, apoptosis, migration, and invasion. In this study, we found an inhibitory effect of CRMP-1 on proliferation, migration, invasion, and an enhanced effect on apoptosis in human trophoblast cell lines HTR-8/SVneo and JEG-3 by MTT assay, colony formation assay, cell viability assay, caspase 3/7 activity assay, scratch wound assay, and Matrigel Transwell assay. Overexpression of CRMP-1 in trophoblast cells led to downregulate expression of matrix metalloproteinase 2 and 9. The expression of CRMP-1 was detected by real-time quantitative polymerase chain reaction and Western blot analysis. Thus, we suggested that CRMP-1 might have implications for the pathogenesis of preeclampsia by regulating the biological behavior of trophoblast cells
%K collapsin response mediator protein 1 (CRMP-1)
%K preeclampsia
%K trophoblast cells
%U https://journals.sagepub.com/doi/full/10.1177/1933719118799214