%0 Journal Article
%T Rapid Alterations in Cerebral White Matter Lipid Profiles After Ischemic
%A Amy Lin
%A Barbara S Stonestreet
%A Gina M Gallucci
%A Ming Tong
%A Suzanne M de la Monte
%A Xiaodi Chen
%J Pediatric and Developmental Pathology
%@ 1615-5742
%D 2019
%R 10.1177/1093526619826721
%X Perinatal ischemia-reperfusion (I/R) injury of cerebral white matter causes long-term cognitive and motor disabilities in children. I/R damages or kills highly metabolic immature oligodendroglia via oxidative stress, excitotoxicity, inflammation, and mitochondrial dysfunction, impairing their capacity to generate and maintain mature myelin. However, the consequences of I/R on myelin lipid composition have not been characterized. This study utilized matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) to assess alterations in cerebral supraventricular white matter myelin lipid profiles in a fetal sheep model of perinatal I/R. Fetal sheep (127 days gestation) were studied after 30 minutes of bilateral carotid artery occlusion followed by 4 (n£¿=£¿5), 24 (n£¿=£¿7), 48 (n£¿=£¿3), or 72 (n£¿=£¿5) hours of reperfusion, or sham treatment (n£¿=£¿5). White matter lipids were analyzed by negative ion mode MALDI-MS. Striking I/R-associated shifts in phospholipid and sphingolipid expression occurred over the 72-hour time course with most responses detected within 4 hours of reperfusion and progressing at the 48- and 72-hour points. I/R decreased expression of phosphatidic acid and phosphatidylethanol amine and increased phosphatidylinositol, sulfatide, and lactosylceramide. Cerebral I/R in mid-gestation fetal sheep causes rapid shifts in white matter myelin lipid composition that may reflect injury, proliferation, or recovery of immature oligodendroglia
%K ischemia reperfusion
%K white matter
%K fetal brain
%K sheep
%K lipidomics
%K mass spectrometry
%U https://journals.sagepub.com/doi/full/10.1177/1093526619826721