%0 Journal Article
%T Investigation of the potential for sera from cattle persistently infected with bovine viral diarrhea virus to generate false
%A David A. Graham
%A Deirdre King
%A Ronan G. O¡¯Neill
%A Tracy A. Clegg
%J Journal of Veterinary Diagnostic Investigation
%@ 1943-4936
%D 2019
%R 10.1177/1040638719830457
%X We investigated the potential for viremic sera from cattle persistently infected with bovine viral diarrhea virus to create false-negative antibody results when testing pools of 10 sera by indirect or blocking ELISAs. Seronegative viremic sera (n = 23) were each added to a series of artificially constructed pools containing various percentages (0¨C90%) of antibody-positive sera, and the resulting pools were assayed for antibody. In all 23 cases, a negative antibody result was obtained in the pool containing no seropositive sera. In contrast, all pools containing ¡İ10% seropositive serum, representing a single seropositive animal in a pool of 10 samples, returned a positive result in both antibody ELISAs. We concluded that the likelihood of a false-negative antibody result occurring as a result of the presence of serum from a viremic animal was low, and therefore did not preclude the use of pooled sera for serosurveillance
%K Antibody
%K bovine viral diarrhea
%K ELISA
%K false negative
%K pooled serum
%K viremic sera
%U https://journals.sagepub.com/doi/full/10.1177/1040638719830457