%0 Journal Article
%T Bovine viral diarrhea in captive Rocky Mountain bighorn sheep associated with administration of a contaminated modified
%A Christie E. Mayo
%A Jennifer H. Kopanke
%A Justin S. Lee
%A Karen A. Fox
%A Kristy L. Pabilonia
%A Lisa L. Wolfe
%J Journal of Veterinary Diagnostic Investigation
%@ 1943-4936
%D 2019
%R 10.1177/1040638718814583
%X In late summer 2017, we observed acute, fatal cases of bovine viral diarrhea in captive Rocky Mountain bighorn sheep (Ovis canadensis canadensis) in Colorado following use of a contaminated modified-live bluetongue virus vaccine. Following vaccination, at least 14 of 28 (50%) vaccinated bighorn sheep developed hemorrhagic diarrhea, and 6 of 28 (21%) vaccinated bighorn sheep died. Autopsy findings were predominantly necroulcerative-to-necrohemorrhagic gastrointestinal lesions. Less frequent lesions included suffusive hemorrhages of serosal surfaces of abdominal viscera, and lymphoid necrosis in gut-associated lymphoid tissues. All of the 6 bighorn sheep that died were positive on real-time PCR (rtPCR) for bovine viral diarrhea virus (BVDV) in multiple tissues. Seroconversion to BVDV-1 and immunohistochemistry for BVDV in affected tissues confirmed rtPCR results. Next-generation sequencing confirmed a match between the infecting strain of BVDV-1b and the contaminated vaccine
%K Bighorn sheep
%K bovine viral diarrhea virus 1
%K Ovis canadensis canadensis
%K vaccine contamination
%U https://journals.sagepub.com/doi/full/10.1177/1040638718814583