%0 Journal Article
%T Regulation of capillary tubules and lipid formation in vascular endothelial cells and macrophages via extracellular vesicle
%A Can Zhou
%A Hui Liu
%A Hui Luo
%A Mao Chen
%A Rongyi Zhang
%A Sha Ke
%A Si Liu
%A Xiao Zhu
%A Ying Yang
%A Zhan Lu
%J Journal of International Medical Research
%@ 1473-2300
%D 2019
%R 10.1177/0300060518809255
%X This study aimed to examine regulation of capillary tubules and lipid formation in vascular endothelial cells and macrophages via extracellular vesicle-mediated microRNA (miRNA)-4306 transfer Whole blood samples (12 mL) were collected from 53 patients, and miR-4306 levels in extracellular vesicles (EVs) were analyzed by reverse transcription-polymerase chain reaction. Human coronary artery vascular endothelial cells (HCAECs) and human monocyte-derived macrophages (HMDMs) were transfected with a scrambled oligonucleotide, an miR-4306 mimic, or an anti-miR-4306 inhibitor. The direct effect of miR-4306 on the target gene was analyzed by a dual-luciferase reporter assay. EV-contained miR-4306 released from HMDMs was significantly upregulated in coronary artery disease. Oxidized low-density lipoprotein (ox-LDL)-stimulated HMDM-derived EVs inhibited proliferation, migration, and angiogenesis abilities of HCAECs in vitro. However, ox-LDL-stimulated HCAEC-derived EVs enhanced lipid formation of HMDMs. The possible mechanism of these findings was partly due to EV-mediated miR-4306 upregulation of the Akt/nuclear factor kappa B signaling pathway. Paracrine cellular crosstalk between HCAECs and HMDMs probably supports the pro-atherosclerotic effects of EVs under ox-LDL stress
%K Extracellular vesicle
%K miR-4306
%K human monocyte-derived macrophage
%K human coronary artery vascular endothelial cell
%K coronary artery disease
%K oxidized low-density lipoprotein
%K lipid
%U https://journals.sagepub.com/doi/full/10.1177/0300060518809255