%0 Journal Article
%T Curcumin regulates the miR
%A Caixia Yi
%A Lianying Yu
%A Lingyu Meng
%A Weihong Sha
%A Wenxia Chen
%A Zhanrong Qiang
%J Journal of International Medical Research
%@ 1473-2300
%D 2019
%R 10.1177/0300060518822213
%X PD98059 is a potent and selective inhibitor of mitogen-activated protein kinase. Substantial preclinical evidence has shown an anti-tumor effect of curcumin on various solid tumors. This study aimed to investigate whether curcumin synergistically acts with PD98059 in exerting anti-gastric cancer effects. The cell counting kit-8 assay was used to detect cell proliferation of the human gastric cancer MGC-803 cell line. Flow cytometry was performed to detect apoptosis. Western blotting was used to detect phosphatase and tensin homolog (PTEN) and phosphorylated Akt (p-Akt) expression levels. Quantitative reverse transcription-polymerase chain reaction was used to determine microRNA-21 (miR-21). A dose of 5 to 40£¿¦ÌM curcumin inhibited proliferation of MGC-803 cells in a dose- and time-dependent manner. A high dose of curcumin strongly inhibited p-Akt protein expression. With increasing curcumin levels, PTEN expression increased and miR-21 levels decreased. These results suggest that curcumin negatively modulated the miR-21/PTEN/Akt pathway. Moreover, after pretreatment with PD98059, cell apoptosis induced by curcumin was significantly increased. Additionally, the inhibitory effects of curcumin on the miR-21/PTEN/Akt pathway were significantly enhanced. PD98059 combined with curcumin may be a potential strategy for managing gastric cancer
%K Curcumin
%K microRNA-21 (miR-21)
%K apoptosis
%K mitogen-activated protein kinase (MAPK) inhibitor
%K stomach neoplasm
%K phosphatase and tensin homolog (PTEN)
%U https://journals.sagepub.com/doi/full/10.1177/0300060518822213