%0 Journal Article
%T Simultaneous detection of multiple pathogens by multiplex PCR coupled with DNA biochip hybridization
%A Bor-Rung Ou
%A Hsiang-Yun Tung
%A Jan-Ying Yeh
%A Ming-Hua Hsu
%A Ming-Shiun Tsai
%A Ping-Hua Tsng
%A Wei-Chen Chen
%A Yeong-Hsiang Cheng
%A Yu-Chuan Liang
%J Laboratory Animals
%@ 1758-1117
%D 2018
%R 10.1177/0023677217718864
%X Traditional serological enzyme-linked immunosorbent assay (ELISA) is routinely used to monitor pathogens during quarantine in most animal facilities to prevent possible infection. However, the ELISA platform is a single-target assay, and screening all targeted pathogens is time-consuming and laborious. In this study, to increase sensitivity and to reduce diagnosis time for high-throughput processes, multiplex PCR and DNA biochip techniques were combined to develop a multi-pathogen diagnostic method for use instead of routine ELISA. Eight primer sets were designed for multiplex PCR to detect genes from seven targeted bacterial and viral pathogens. DNA¨CDNA hybridization was conducted on a biochip following the multiple PCR analysis. Using this method, a total of 24 clinical samples were tested, and the result showed that not only single infection but also co-infection by multi-pathogens can be detected. In conclusion, multiplex PCR coupled with a DNA biochip is an efficient method for detecting multi-pathogens in a reaction. This platform is a useful tool for quarantine services and disease prevention in animal facilities
%K ELISA
%K pathogen
%K multiplex PCR
%K DNA biochip
%U https://journals.sagepub.com/doi/full/10.1177/0023677217718864