%0 Journal Article
%T Analytical validation of fecal 3
%A Jan S. Suchodolski
%A Jonathan A. Lidbury
%A Jˋrg M. Steiner
%A Naris Thengchaisri
%A Panpicha Sattasathuchana
%J Journal of Veterinary Diagnostic Investigation
%@ 1943-4936
%D 2019
%R 10.1177/1040638719831340
%X Studies that have used serum 3-bromotyrosine (3-BrY) to investigate eosinophil activation in dogs have found elevated 3-BrY levels in clinical patients with chronic enteropathy (CE). To our knowledge, a method to measure 3-BrY concentrations in feces has not been reported. We developed and analytically validated an electron ionization gas chromatography每mass spectrometry method to measure fecal 3-BrY concentrations in dogs. The mean and maximum fecal 3-BrY concentrations in healthy dogs (n = 40) and dogs with CE (n = 40) over 3 consecutive days were compared. Analytical validation had a limit of blank and a limit of detection of 2.5 and 3.7ˋmmol/g of feces, respectively. The mean coefficients of variation for precision and reproducibility for 3-BrY were 11.2% (range: 7.5每14.2%) and 10.1% (4.8每15.2%), respectively. The ranges of observed-to-expected ratios for linearity and accuracy were 81.3每125% and 85.4每120%, respectively. The reference intervals for mean and maximum fecal 3-BrY concentrations in 40 healthy dogs were 3.7每23.0 and 3.7每37.8ˋmmol/g of feces. Mean and maximum fecal 3-BrY concentrations in dogs with CE were significantly higher than those of healthy dogs (p < 0.001). Further research is warranted to determine the clinical usefulness of fecal 3-BrY concentrations in dogs with CE
%K 3-bromotyrosine
%K chronic enteropathy
%K dogs
%K eosinophils
%K feces
%K validation studies
%U https://journals.sagepub.com/doi/full/10.1177/1040638719831340