%0 Journal Article
%T Expansion of mouse hematopoietic stem/progenitor cells in three
%A Chiaki Sato
%A Hirotoshi Miyoshi
%A Misa Morita
%A Yuichiro Shimizu
%J The International Journal of Artificial Organs
%@ 1724-6040
%D 2019
%R 10.1177/0391398819827596
%X With the aim of establishing an effective method to expand hematopoietic stem/progenitor cells for application in hematopoietic stem cell transplantation, we performed ex vivo expansion of hematopoietic stem/progenitor cells derived from mouse fetal liver cells in three-dimensional cocultures with stromal cells. In these cocultures, stromal cells were first cultured within three-dimensional scaffolds to form stromal layers and then fetal liver cells containing hematopoietic cells were seeded on these scaffolds to expand the hematopoietic cells over the 2£¿weeks of coculture in a serum-containing medium without the addition of cytokines. Prior to coculture, stromal cell growth was suppressed by treatment with the DNA synthesis inhibitor mitomycin C, and its effect on hematopoietic stem/progenitor cell expansion was compared with that in control cocultures in which fetal liver cells were cocultured with three-dimensional freeze-thawed stromal cells. After coculture with mitomycin C-treated stromal cells, we achieved a several-fold expansion of the primitive hematopoietic cells (c-kit+ hematopoietic progenitor cells >7.8-fold, and CD34+ hematopoietic stem/progenitor cells >3.5-fold). Compared with control cocultures, expansion of hematopoietic stem/progenitor cells tended to be lower, although that of hematopoietic progenitor cells was comparable. Thus, our results suggest that three-dimensional freeze-thawed stromal cells have higher potential to expand hematopoietic stem/progenitor cells compared with mitomycin C-treated stromal cells
%K Cryopreservation
%K ex vivo expansion
%K hematopoietic stem/progenitor cell
%K mitomycin C
%K stromal cell
%K three-dimensional culture
%U https://journals.sagepub.com/doi/full/10.1177/0391398819827596