%0 Journal Article
%T Dietary fish oil supplementation alters liver gene expressions to protect against LPS
%A Huiling Zhu
%A Jing Zhang
%A Xin Xu
%A Yang Wang
%A Yongqing Hou
%A Yulan Liu
%J Innate Immunity
%@ 1753-4267
%D 2019
%R 10.1177/1753425918821420
%X Here, the potential mechanisms of the protective effects of fish oil against LPS-induced liver injury in a piglet model were investigated by using RNA sequencing. Twenty-four piglets were used in a 2£¿¡Á£¿2 factorial design, and the main factors included diet (5% corn oil or 5% fish oil) and immunological challenge (LPS or saline, on d 19). All piglets were slaughtered at 4 h after challenge, and liver samples were collected. Fish oil improved liver morphology and reduced TNF-¦Á, IL-1¦Â and IL-6 productions after LPS challenge. RNA sequencing analysis showed fish oil had significant effect on the expressions of genes involved in immune response during LPS-induced inflammation. Selected gene expression changes were validated using quantitative RT-PCR. Fish oil reduced the expressions of pro-inflammatory genes IL1R1, IL1RAP, CEBPB and CRP, and increased that of anti-inflammatory genes IL-18BP, NFKBIA, IFIT1, IFIT2 and ATF3. Moreover, fish oil restored the expressions of some lipid metabolism-related genes, such as ACAA1, ACACA, ACADS and ACADM, which were only decreased in pigs fed a corn oil diet after LPS challenge. Our RNA sequencing reveals novel gene-nutrient interactions following fish oil supplementation and evoked inflammation, which add to the current understanding of the benefits of n-3 polyunsaturated fatty acids against liver injury
%K Fish oil
%K inflammation
%K lipopolysaccharide
%K liver
%K RNA sequencing
%K pig
%U https://journals.sagepub.com/doi/full/10.1177/1753425918821420