%0 Journal Article
%T Cerium
%A Bin Yu
%A Haibo Xiang
%A Hong Chang
%A Mei Tu
%A Shenyu Yang
%A Xianrong Zhang
%A Yu Wang
%J Journal of Biomaterials Applications
%@ 1530-8022
%D 2019
%R 10.1177/0885328219849712
%X Bone substitute offers a promising strategy for the reconstruction of large bone defects. It may be feasible to develop a better substitute material by enhancing the osteoinductivity and delaying the degradation of ¦Á-calcium sulfate hemihydrate. In this study, nano cerium oxide was incorporated with ¦Á-calcium sulfate hemihydrate at weight ratios of 5 and 10% to form nano cerium oxide-containing ¦Á-calcium sulfate hemihydrate. Extracts from ¦Á-calcium sulfate hemihydrate, 5 and 10% nano cerium oxide-containing ¦Á-calcium sulfate hemihydrate were prepared for in vitro treatment of cells. After critical bone defects were made on the left tibia of male Sprague¨CDawley rats, the animals were randomly divided into four groups subjected to the filling with nothing (blank), ¦Á-calcium sulfate hemihydrate (control), 5 and 10% nano cerium oxide-containing ¦Á-calcium sulfate hemihydrate. Extraction from 5% nano cerium oxide-containing ¦Á-calcium sulfate hemihydrate composite stimulated cells proliferation and increased cell migration and mRNA expression of osteoblastic marker genes in primary bone marrow stromal cells. X-ray and micro-computed tomography analyses showed increased bone formation and reduced degradation, and histological analysis showed mineralized staining on the boundary area between composites and bone marrow in the nano cerium oxide-containing ¦Á-calcium sulfate hemihydrate group. Immunostaining of osteocalcin confirmed the increased bone formation in the nano cerium oxide-containing ¦Á-calcium sulfate hemihydrate group. Nano cerium oxide-containing ¦Á-calcium sulfate hemihydrate composite with 5% w/w nano cerium oxide showed a better capacity of enhancing osteogenesis and bone regeneration model than 10% cerium oxide-containing ¦Á-calcium sulfate hemihydrate composite. The findings suggested that the nano cerium oxide-containing ¦Á-calcium sulfate hemihydrate might be a potential material for bone substitutes since it may promote local bone regeneration
%K Cerium
%K calcium sulfate
%K bone substitute
%K bone regeneration
%K bone marrow stromal cells
%U https://journals.sagepub.com/doi/full/10.1177/0885328219849712