%0 Journal Article
%T A novel high
%A Brian G Keevil
%A Elizabeth S Baranowski
%A Joanne E Adaway
%A Lina Schiffer
%A Wiebke Arlt
%J Annals of Clinical Biochemistry
%@ 1758-1001
%D 2019
%R 10.1177/0004563218780904
%X Liquid chromatography tandem mass spectrometry (LC-MS/MS) enables specific and sensitive quantification of steroids with a high throughput. Saliva sampling is advantageous for multisample profiling over longer periods of time, as it is non-invasive, cheap, can be carried out at home and does not require the attendance of clinical personnel. We developed a rapid LC-MS/MS for the measurement of salivary progesterone, frequently assessed as ovulation marker in patients desiring fertility. Samples (300 ¦ÌL) were prepared by supported liquid extraction using dichloromethane and were reconstituted in 40% methanol. Chromatography was performed using a C8 column with a water/methanol gradient containing 0.1% formic acid and 2 mmol/L ammonium acetate. Quantification was performed with a Waters TQ-S mass spectrometer. Total run time was 5.5 min. The lower limit of quantification was 20£¿pmol/L (1.2 fmol on column). Inter- and intra-assay comparison showed coefficients of variation and bias between measured and nominal concentrations of less than 11%. Mean recovery was 91%. Interference with a large set of natural and synthetic steroids was excluded. The assay was successfully applied to measure progesterone variation during the menstrual cycle (n£¿=£¿9) and diurnal variations during luteal phase (n£¿=£¿7) in regularly cycling women. We present a novel LC-MS/MS assay for the determination of salivary progesterone with high-throughput potential. The applicability of the assay for progesterone profiling during the menstrual cycle is demonstrated
%K Progesterone
%K saliva
%K liquid chromatography tandem mass spectrometry
%U https://journals.sagepub.com/doi/full/10.1177/0004563218780904