%0 Journal Article
%T Determination of total, free and esterified short
%A Akiko Tamakoshi
%A Hitoshi Chiba
%A Rojeet Shrestha
%A Shu-Ping Hui
%A Yaoyao Zhao
%A Yue Wu
%A Yusuke Miura
%A Zhen Chen
%A Zijun Gao
%J Annals of Clinical Biochemistry
%@ 1758-1001
%D 2019
%R 10.1177/0004563218801393
%X Short-chain fatty acids are primarily absorbed through the portal vein during lipid digestion, which is utilized as the energy source, as well as prevent type 2 diabetes and some cancers. However, reports on the determination of these short-chain fatty acids in human serum are limited. Blood samples from human subjects (n£¿=£¿547, male/female£¿=£¿246/301, age 58.85£¿¡À£¿12.57) were collected. Saponification was applied to obtain total fatty acid. After derivatization by 2-nitrophenylhydrazine, fatty acid 4:0 and fatty acid 6:0 were measured by liquid chromatography-mass spectrometry. The developed method exhibited good linearity (R2£¿=£¿0.9996 for both). All the coefficients of variation of reproducibility and accuracy for fatty acid 4:0 and fatty acid 6:0 ranged 3.0%£¿6.1%, with the average recoveries of 87.8%£¿102.4% and 92.2%£¿98.2%, respectively. In all the samples, the concentration of fatty acid 4:0 (162.4£¿¡À£¿76.4 ¦Ìmol/L) was significantly higher than fatty acid 6:0 (2.0£¿¡À£¿2.5 ¦Ìmol/L, P£¿<£¿0.001). Furthermore, the esterified form was predominant in both fatty acid 4:0 and fatty acid 6:0 (98.2% and 82.4% of total fatty acids, respectively). Besides, short-chain fatty acids showed no significant differences with regard to sex or age differences. This developed liquid chromatography-mass spectrometry method is convenient and reliable, which might be useful for monitoring the variations of short-chain fatty acids in blood
%K 0)
%K caproic acid (FA 6:0)
%K LC-MS/MS
%K serum
%U https://journals.sagepub.com/doi/full/10.1177/0004563218801393