%0 Journal Article
%T Augmented tumor accumulation and photothermal ablation using gold nanoparticles with a particular cellular entry orientation
%A Eun Seong Lee
%A Gwang Jin Noh
%A Hongsuk Park
%J Journal of Bioactive and Compatible Polymers
%@ 1530-8030
%D 2018
%R 10.1177/0883911518809112
%X Gold nanoparticles with various functionalities have served as potential tools in nanotechnology for tumor ablation. In this work, we seek to design and develop gold nanoparticle with poly(ethylene glycol)-containing dopamine (hereafter termed as AuND), and to synthesize the AuND with one-sided Tat peptide expression ([email protected]). We demonstrate the tumor cell-targeting ability on the basis of anti-nonspecific cell binding of [email protected] and determine how the chemically modified gold nanoparticle¨Cbased product affects photothermal tumor therapy in vitro and in vivo. The [email protected] with a particular cellular entry orientation¨Cinduced delayed endocytosis, which is advantageous for enhanced permeability and retention effect-based tumor accumulation. This is because the slower cellular interaction of [email protected] allows it to have the time to be transported to and bind to the tumor site. In tumor cell lines, [email protected] showed a lower cellular uptake than gold nanoparticles with full-sided Tat peptide expression ([email protected]) in the early period (after its in vitro and in vivo administration), but the cellular internalization rate of [email protected] caught up with that of [email protected] in the late period. Importantly, the delayed cellular internalization feature of [email protected] resulted in efficient tumor accumulation in tumor-bearing mice, because the time interval provided [email protected] more chances not to bind to any cells, but to enter tumor cells, leading to selective photothermal tumor ablation. These data suggest that gold nanoparticles with a particular cellular entry orientation can be further explored as a potential photothermal therapeutic agent and as a strategy to treat tumors
%K Gold nanoparticles
%K one-sided Tat peptide
%K cellular entry orientation
%K photothermal tumor ablation
%K enhanced permeability and retention
%U https://journals.sagepub.com/doi/full/10.1177/0883911518809112