%0 Journal Article
%T In vitro gene expression and detergent
%A David Thomas T Gonzales
%A Giorgio Fracasso
%A T-Y. Dora Tang
%A Yvonne K£¿rner
%J Experimental Biology and Medicine
%@ 1535-3699
%D 2019
%R 10.1177/1535370218820290
%X In situ generation of proton gradients across membranes is a key component for energy generation within cells and is therefore an important feature for the design of energy converting artificial cells. Here, we establish a stepwise method for the in vitro expression and detergent-free reconstitution of proteorhodopsin into the membrane of lipid vesicles. This represents a novel technique towards the bottom-up synthesis of energy-generating artificial cells. Our results offer the potential for straightforward, additive-free, and molecularly simple routes to building complex bioreactors based on in vitro transcription¨Ctranslation systems and lipid vesicles
%K Lipids synthetic biology
%K cell-free protein expression
%K protein reconstitution
%K artificial cells
%K proteorhodopsin
%U https://journals.sagepub.com/doi/full/10.1177/1535370218820290