%0 Journal Article
%T A Biocontainment Procedure for Intravital Microscopy of High
%A Benjamin Fontes
%A Kelsey A. Haugh
%A Pradeep D. Uchil
%A Ruoxi Pi
%A Walther Mothes
%J Applied Biosafety
%@ 2470-1246
%D 2018
%R 10.1177/1535676018785177
%X Intravital multiphoton microscopy offers valuable spatio-temporal insight into studies of pathogen-host interactions. Although well established for studies with low-risk biohazard studies, the complexity of the intravital imaging technique has been difficult for applications using high-risk pathogens. Here, we delineate our standard operating procedure (SOP) for intravital microscopy studies of Risk Groups 2 and 3 pathogens. Using carefully designed protocols adapted to the pathogens under study, we present strategies that allow application of this complex technique to high-risk studies while maximizing protection of personnel and animals in the facility. While we describe our SOP for studies of HIV in vivo, these strategies serve as a guideline for designing SOPs for a wide variety of pathogen studies employing intravital imaging
%K intravital microscopy
%K 2-photon microscopy
%K Risk Group 3 pathogens
%K BSL-3 containment
%K small animals
%K HIV
%U https://journals.sagepub.com/doi/full/10.1177/1535676018785177