%0 Journal Article
%T How to Avoid False
%A Armen V. Gyulkhandanyan
%A John Freedman
%A Valery Leytin
%J Clinical and Applied Thrombosis/Hemostasis
%@ 1938-2723
%D 2018
%R 10.1177/1076029618778140
%X Platelets may selectively execute apoptosis (PL-Apo), activation (PL-Act), and both or no responses when exposed to different chemical agents, shear stresses, and stored under blood banking conditions. Appropriate diagnosis of PL-Apo is an important issue of platelet physiology investigations. However, in diagnosing PL-Apo, there is a risk of a false-negative or false-positive diagnosis. The goal of the current review is to present recommendations that may help to avoid incorrect PL-Apo diagnosis. Analyzing reported studies, we recommend (1) using platelet-rich plasma rather than isolated platelets to minimize artificial stimulation of PL-Apo during platelet isolation, (2) using established optimal conditions for stimulation of PL-Apo and/or PL-Act, (3) using a panel of PL-Apo and PL-Act markers, and (4) appropriate positive and negative controls for quantification of PL-Apo and PL-Act responses
%K platelet apoptosis
%K platelet activation
%K diagnosis of platelet apoptosis
%U https://journals.sagepub.com/doi/full/10.1177/1076029618778140