%0 Journal Article
%T Structure¨Cproperty relation of porous poly (l
%A Harish Chinnasami
%A Jeff Gimble
%A Ram V Devireddy
%J Journal of Bioactive and Compatible Polymers
%@ 1530-8030
%D 2018
%R 10.1177/0883911518758354
%X Thermally induced phase separation method was used to make porous three-dimensional poly (l-lactic acid) scaffolds. The effect of imposed thermal profile during freezing of the poly (l-lactic acid) in dioxane solution on the scaffold was characterized by their micro-structure, porosity (%), pore sizes¡¯ distribution, and mechanical strength. The porosity (%) decreased considerably with increasing concentrations of poly (l-lactic acid) in the solution, while a decreasing trend was observed with increasing cooling rates. The mechanical strength increases with increase in poly (l-lactic acid) concentration and also with increase in the cooling rate for both types of solvents. Therefore, mechanical strength was increased by higher cooling rates while the porosity (%) remained relatively consistent. Scaffolds made using higher concentrations of poly (l-lactic acid; 7% and 10% w/v) in solvent showed better mechanical strength which improved relatively with increasing cooling rates (1¡ãC¨C40¡ãC/min). This phenomenon of enhanced structural integrity with increasing cooling rates was more prominent in scaffolds made from higher initial poly (l-lactic acid) concentrations. Human adipose¨Cderived stem cells were cultured on these scaffold (7% and 10% w/v) prepared by thermally induced phase separation at all cooling rates to measure the cell proliferation efficiency as a function of their micro-structural properties. Mean pore sizes played a crucial role in cell proliferation than percent porosity since all scaffolds were >88% porous. The viability percent of human adipose tissue¨Cderived adult stem cells increased consistently with longer periods of culture. Thus, poly (l-lactic acid) scaffolds prepared by thermally controlled thermally induced phase separation method could be a prime candidate for making ex vivo tissue-engineered grafts for surgical implantation
%K Thermally induced phase separation
%K poly (l-lactic acid)
%K dioxane
%K ethanol
%K human adipose stem cells
%K scanning electron microscopy
%K compressive modulus and controlled rate freezing
%U https://journals.sagepub.com/doi/full/10.1177/0883911518758354