%0 Journal Article
%T Identification of specific integrin cross
%A Fumihiko Katagiri
%A Kentaro Hozumi
%A Motoyoshi Nomizu
%A Sayaka Enomoto
%A Yamato Kikkawa
%A Yui Teranishi
%J Journal of Biomaterials Applications
%@ 1530-8022
%D 2019
%R 10.1177/0885328218823457
%X Extracellular matrix molecules are recognized by several integrin subtypes, making identification of cross-talk among different integrin subtypes difficult. Here, we evaluated the cross-talk of integrin subtypes using four different integrin-binding peptides (FIB1; integrin ¦Áv¦Â3/¦Á5¦Â1, A2G10; integrin ¦Á6¦Â1, EF1zz; integrin ¦Á2¦Â1, or 531; integrin ¦Á3¦Â1) derived from extracellular matrix molecules. Various combinations of two different integrin-binding peptides were mixed and conjugated on a chitosan matrix at various molar ratios and were evaluated for cell attachment activity. FIB1/A2G10 (molar ratio 5:5; total 10 nmol/well)-chitosan matrix significantly enhanced cell attachment activity compared with sum of the cell attachment activity on FIB1 (5 nmol/well)-chitosan matrices and A2G10 (5 nmol/well)-chitosan matrices, respectively. However, none of the other peptides showed a significant activity change when they were mixed and conjugated on a chitosan matrix. We investigated the mechanisms of this enhancement. FIB1/A2G10 (8:2 or 6:4)-chitosan matrix increased the cell spreading, phosphorylation of focal adhesion kinase at Y397, and slightly decreased phosphorylation of caveolin-1 at Y14 in fibroblasts compared with FIB1-chitosan and A2G10-chitosan matrices. These results indicate that FIB1/A2G10 (8:2 or 6:4)-chitosan matrix synergistically enhances cell attachment, suggesting that integrins ¦Áv¦Â3/¦Á5¦Â1 and ¦Á6¦Â1 are involved in a cross-talk and synergistically enhance cell attachment. These findings also suggest that the mixed peptide-chitosan matrix system can regulate the ratio of two different peptides and is useful for evaluating cellular functions through receptor-specific cross-talk. Further, FIB1/A2G10 (8:2 or 6:4)-chitosan matrix could be a useful material for tissue engineering
%K Extracellular matrix
%K chitosan
%K cross-talk
%K integrin
%K peptide
%K scaffold
%K cell adhesion
%U https://journals.sagepub.com/doi/full/10.1177/0885328218823457