%0 Journal Article
%T Enhanced antitumor activity of epigallocatechin gallate每conjugated dual
%A Baki Hazer
%A Ebru Kilicay
%A Emir Baki Denkbas
%A Pˋnar Alpaslan
%A Zeynep Karahaliloˋlu
%J Journal of Bioactive and Compatible Polymers
%@ 1530-8030
%D 2018
%R 10.1177/0883911517710811
%X The development of novel combination anticancer drug delivery systems is an important step to improve the effectiveness of anticancer treatment in metastatic breast cancer and to overcome increased toxicity of the currently used combination treatments. The aim of this study was to assess efficient targeting, therapeutic efficacy, and bioavailability of a combination of drugs (curcumin and 汐-tocopheryl succinate) loaded polystyrene每polysoyaoil每diethanol amine nanoparticles. Polystyrene每polysoyaoil每diethanol amine nanoparticles encapsulating two drugs, individually or in combination, were prepared by double-emulsion solvent evaporation method, resulting in particle size smaller than 250ˋnm with a surface negative charge between ˋ30 and ˋ40ˋmV. Entrapment efficiency of curcumin and 汐-tocopheryl succinate in the epigallocatechin gallate每conjugated dual-drug-loaded nanoparticles was found to be 68% and 80%, respectively. The release kinetics of curcumin and 汐-tocopheryl succinate from the nanoparticles exhibited a gradual and continuous profile followed by an initial burst behavior with a release over 20ˋdays in vitro. Next, we have investigated the anticancer activity of nanoparticles encapsulating both the drugs and individually drug in human breast cancer cells (MDA-MB-231) using double-staining-based cell death analysis, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assessment of cytotoxicity and flow cytometer. In vitro cytotoxicity studies revealed that epigallocatechin gallate每汐-tocopheryl succinate/curcumin每polystyrene每polysoyaoil每diethanol amine nanoparticles are more potent than the corresponding 汐-tocopheryl succinate/curcumin每polystyrene每polysoyaoil每diethanol amine nanoparticles and their single-drug-loaded forms and show a synergistic and breast tumor targeting function. Thus, here, we propose epigallocatechin gallate每conjugated curcumin and 汐-tocopheryl succinate每loaded polystyrene每polysoyaoil每diethanol amine nanoparticles which effectively inhibit tumor growth and reduce toxicity compared to single-drug chemotherapy
%K Curcumin
%K drug delivery
%K epigallocatechin gallate
%K 汐-tocopheryl succinate
%K polystyrene
%K soya oil
%U https://journals.sagepub.com/doi/full/10.1177/0883911517710811