%0 Journal Article
%T Therapeutic Efficacy of Spherical Aggregated Human Bone Marrow每Derived Mesenchymal Stem Cells Cultured for Osteochondral Defects of Rabbit Knee Joints
%A Byung Hoon Lee
%A Eun Ju Lee
%A Jong Nam Park
%A Joon Ho Wang
%A Young Wan Moon
%J The American Journal of Sports Medicine
%@ 1552-3365
%D 2018
%R 10.1177/0363546518780991
%X Engraftment and longevity of transplanted cells are crucial for stem cell每based cartilage treatment. To determine whether cultured spherical cell masses of human bone marrow每derived mesenchymal stem cells (hBM-MSCs) could improve engraftment at defect sites and to examine their corresponding effects on osteochondral regeneration. Controlled laboratory study. A cylindrical osteochondral defect (5 mm wide ℅ 5 mm deep) was created in trochlear grooves of rabbit knees. The single-cell type of hBM-MSCs with fibrin glue, the spherical type of hBM-MSCs with fibrin glue, and cell-free fibrin glue (control) were each implanted into osteochondral defect sites. A total of 18 rabbit knees were randomly assigned to 1 of the 3 groups (3 rabbits per group). Animals were sacrificed at 6 and 12 weeks after transplantation. Repaired tissues were evaluated via gross examination, histologic examination, and immunofluorescence analysis. Transplantation with spherical hBM-MSCs exhibited superior overall osteochondral restoration when compared with the single-type group, as evidenced by well-ordered mature collagen fibrils produced during subchondral bone formation in the zonation phenomenon. Immunofluorescence analysis of osteochondral defect areas with human-specific antigen revealed a larger number of mesenchymal stem cells in the spherical-type group than the single cell每type group. Transplantation of spherical hBM-MSCs was better than single cells from monolayer culture in improving osteochondral regeneration. The findings demonstrate a simple strategy for enhancing the potency of stem cells required for restoration of osteochondral defects. Furthermore, this strategy may be implemented with other types of stem/progenitor cell每based therapies
%K osteochondral defect
%K cell transplantation
%K mesenchymal stem cells
%K sphere formation
%U https://journals.sagepub.com/doi/full/10.1177/0363546518780991