%0 Journal Article
%T A two
%A Atsushi Nagasaka
%A Hitoshi Chiba
%A Masato Fujikawa
%A Norio Wada
%A Shu-Ping Hui
%A Toshihiro Sakurai
%A Yasuki Ito
%A Yuji Takahashi
%J Annals of Clinical Biochemistry
%@ 1758-1001
%D 2019
%R 10.1177/0004563218795212
%X Apolipoprotein E-containing high-density lipoprotein shows antiatherogenic properties in vitro. There is a need for a homogeneous assay to determine the concentration of apolipoprotein E-containing high-density lipoprotein for in vivo studies. In the proposed homogeneous assay, lipoproteins other than apolipoprotein E-containing high-density lipoprotein were eliminated in the first step. Apolipoprotein E-containing high-density lipoprotein-cholesterol was measured in the second step. The control study used a 13% polyethylene glycol precipitation assay (control assay). The homogeneous assay showed good performance in validation studies. In subjects with normal liver function (nˋ=ˋ78), a significant correlation was found between the control assay and the homogeneous assay (rˋ=ˋ0.824). Serum apolipoprotein E-containing high-density lipoprotein cholesterol concentrations, determined by the control assay and the homogeneous assay, respectively, were 0.05 (0.04每0.10) (median [25th每75th percentile]) mmol/L and 0.10 (0.06每0.13) mmol/L for healthy individuals (nˋ=ˋ12), and 0.03 (0.01每0.13) mmol/L and 0.02 (0.01每0.02) mmol/L for patients with cholestasis (nˋ=ˋ6). The results indicate that the homogeneous assay recovers cholesterol contained in physiological apolipoprotein E-containing high-density lipoprotein, but not in pathological apolipoprotein E-containing high-density lipoprotein from cholestatic patients. The proposed two-step homogeneous assay enables selective measurement of physiological apolipoprotein E-containing high-density lipoprotein cholesterol in common autoanalysers. This assay might uncover a role for apolipoprotein E-containing high-density lipoprotein in physiological conditions
%K Lipids
%K analytical systems
%K enzymatic methods
%U https://journals.sagepub.com/doi/full/10.1177/0004563218795212