%0 Journal Article
%T The effects of cigarette smoking and smoking cessation on high
%A Hong-Ying Chen
%A Lian-Feng Chen
%A Shi-Cheng Li
%A Wei Wang
%A Xiao-Wei Yan
%A Yu Wang
%J Annals of Clinical Biochemistry
%@ 1758-1001
%D 2019
%R 10.1177/0004563218788386
%X Smoking cessation was associated with improved prognosis of coronary artery disease. This study was designed to investigate the effect of smoking cessation on high-density lipoprotein functionality in coronary artery disease patients. In this prospective, randomized and parallel controlled study, coronary artery disease smokers (n£¿=£¿28) and healthy smokers (n£¿=£¿30) were divided into smoking cessation group and continuous smoking group, respectively. Blood samples were collected before and after three-month smoking cessation. Plasma high-density lipoprotein was isolated by density gradient centrifugation. The ability of high-density lipoprotein against copper-induced oxidation of lipoprotein was determined to evaluate the antioxidative property of high-density lipoprotein, and the macrophage migration inhibited by high-density lipoprotein was tested to identify the antichemotactic property of high-density lipoprotein. High-density lipoprotein-induced macrophage cholesterol efflux was measured by fluorescence spectrometry using NBD cholesterol analogue. Healthy non-smoking volunteers were enrolled as the baseline control. The baseline antioxidative, antichemotactic ability of high-density lipoprotein and high-density lipoprotein-induced cellular cholesterol efflux in coronary artery disease smokers and healthy smokers were significantly attenuated when compared with those in healthy non-smokers. After three-month smoking cessation, both the antioxidative ability and antichemotactic ability of high-density lipoprotein were improved significantly in coronary artery disease smokers. However, high-density lipoprotein-induced cellular cholesterol efflux was not increased by smoking cessation. In in vitro experiments, carbon monoxide reduced the antioxidative ability and nicotine enhanced the antichemotactic ability of high-density lipoprotein. Smoking cessation is an effective measure to improve high-density lipoprotein functions in coronary artery disease smokers. Our study re-emphasizes the importance of smoking cessation in the secondary prevention of coronary artery disease
%K Coronary artery disease
%K smoking cessation
%K high-density lipoprotein
%K antioxidation
%K antichemotaxis
%K cholesterol efflux
%U https://journals.sagepub.com/doi/full/10.1177/0004563218788386