%0 Journal Article
%T Characterization and biological properties of a novel synthesized silicon
%A Kamal Asadipour
%A Masoud Hafezi-Ardakani
%A Mohammad Sadegh Nourbakhsh
%A Nader Nezafati
%A Saleh Bohlooli
%J The International Journal of Artificial Organs
%@ 1724-6040
%D 2019
%R 10.1177/0391398818806159
%X In the present study, the effect of adding different concentrations of silicon on physical, mechanical and biological properties of a synthesized aqueous precipitated eggshell-derived hydroxyapatite (e-HA) was evaluated. No secondary phases were detected by X-ray diffraction for the specimens e-HA and e-HA containing silicon (Si-e-HAs) before and after heating at 1200¡ãC. A reduction in the crystallite size and a-axis as well as an increase in c-axis was occurred when silicon replacement was happened in the structure of e-HA. The presence of Si-O vibrations and carbonate modes for Si-e-HAs was confirmed by Fourier transform infrared spectroscopy analysis. The range of porosity and density was varied from 25% and 2.4£¿g£¿cm¨C3 to 7% and 2.8£¿g£¿cm¨C3 for e-HA and Si-e-HAs. The values of Young¡¯s modulus (E) and compressive strength were varied for e-HA and Si-e-HAs. The porous structure of the samples was reduced when they were heated as e-HA kept the porous microstructure containing some dense areas and Si-e-HAs possessed a rough surface including slight levels of microporosity. The acellular in vitro bioactivity represented different apatite morphologies for e-HA and Si-e-HAs. The G-292 osteoblastic cells were stretched well on the surface with polygon-shaped morphology for 0.8Si-e-HA after 7£¿days of culture. According to MTT assay and alkaline phosphatase test, the maximum cell activity was related to 0.8Si-e-HA. The minimum inhibitory concentration for 0.8Si-e-HA and e-HA was estimated to be about 3.2 and 4.4£¿mg/mL, respectively. In overall, the sample 0.8Si-e-HA exhibited a higher bacteriostatic effect than e-HA against gram-negative bacterial strain Escherichia coli
%K Silicon
%K eggshell
%K hydroxyapatite
%K bioactivity
%K cell behaviour
%K antibacterial test
%U https://journals.sagepub.com/doi/full/10.1177/0391398818806159