%0 Journal Article
%T Immobilization of bone morphogenetic protein
%A Cheng-Hsin Cheng
%A Chun-Hsu Yao
%A Kuo-Yu Chen
%A Yi-Hui Lai
%A Yi-Wen Chen
%J Journal of Biomaterials Applications
%@ 1530-8022
%D 2019
%R 10.1177/0885328218820636
%X Bone scaffold surface characterization is important for improving cell adhesion, migration, and differentiation. In this study, bone morphogenetic protein-2 (BMP-2) was immobilized to the surface of the gelatin/hydroxyapatite composite using avidin¨Cbiotin binding system to produce a bone-tissue engineering scaffold. Firstly, hydroxyapatite particles reacted with hexamethylene diisocyanate and then the terminal group was converted into a primary amine group. Avidin was then immobilized on the surfaces of hydroxyapatite particles using N-ethyl-N¡ä-(3-(dimethylamino)propyl) carbodiimide and N-hydroxysuccinimide as coupling agents. Gelatin was blended with avidin-modified hydroxyapatite and pure hydroxyapatite to obtain gelain/hydroxyapatite composite. The composite was then cross-linked with glutaraldehyde. Finally, biotin-conjugated BMP-2 was immobilized on the surface of the composite via avidin¨Cbiotin binding. In vitro study indicated that BMP-2-immobilized composite film had a higher ALP activity than that composite film without BMP-2. The composite scaffolds were then implanted into rabbit skulls to check bone-tissue regeneration. Ultrasound and micro-CT scans demonstrated that neovascularization and new bone formation in the BMP-2-immobilized composite scaffolds were higher than those in composite scaffolds without BMP-2. Histological evaluation result was similar to that of the micro-CT. Therefore, the surface immobilization of BMP-2 could effectively improve osteogenesis in the gelatin/hydroxyapatite composite scaffold
%K Bone morphogenetic protein-2
%K gelatin
%K hydroxyapatite
%K avidin
%K bone-tissue regeneration
%U https://journals.sagepub.com/doi/full/10.1177/0885328218820636