%0 Journal Article
%T Rapid Prototyping Platform for Saccharomyces cerevisiae Using Computer
%A A. Foster
%A D. W. McClymont
%A G-O. F. Gowers
%A L. Suckling
%A P. D. Rajakumar
%A P. S. Freemont
%A R. I. Kitney
%A T. Ellis
%J SLAS TECHNOLOGY: Translating Life Sciences Innovation
%@ 2472-6311
%D 2019
%R 10.1177/2472630318798304
%X Biofoundries have enabled the ability to automate the construction of genetic constructs using computer-aided design. In this study, we have developed the methodology required to abstract and automate the construction of yeast-compatible designs. We demonstrate the use of our in-house software tool, AMOS, to coordinate with design software, JMP, and robotic liquid handling platforms to successfully manage the construction of a library of 88 yeast expression plasmids. In this proof-of-principle study, we used three fluorescent genes as proxy for three enzyme coding sequences. Our platform has been designed to quickly iterate around a design cycle of four protein coding sequences per plasmid, with larger numbers possible with multiplexed genome integrations in Saccharomyces cerevisiae. This work highlights how developing scalable new biotechnology applications requires a close integration between software development, liquid handling robotics, and protocol development
%K high-throughput yeast transformation
%K design of eperiment (DOE)
%K yeast toolkit (YTK)¡ªGolden Gate
%K acoustic liquid handling workcell
%K synthetic biology
%U https://journals.sagepub.com/doi/full/10.1177/2472630318798304