%0 Journal Article
%T Roles of Progesterone Receptor Membrane Component 1 in Oxidative Stress¨CInduced Aging in Chorion Cells
%A Amy P. Murtha
%A Liping Feng
%A Terrence K. Allen
%A William P. Marinello
%J Reproductive Sciences
%@ 1933-7205
%D 2019
%R 10.1177/1933719118776790
%X Oxidative stress¨Cmediated fetal membrane cell aging is activated prematurely in preterm premature rupture of membranes (PPROMs). The mechanism of this phenomenon is largely understudied. Progesterone receptor membrane component 1 (PGRMC1) has been recognized as a potential protective component for maintaining fetal membrane integrity and healthy pregnancies. We aimed to investigate the effects of oxidative stress (represented by hydrogen peroxide [H2O2]) on fetal membrane and chorion cell senescence, p38 mitogen-activated protein kinase (MAPK) phosphorylation, and sirtuin 3 (SIRT3) and to examine the roles of PGRMC1 in these effects. Following serum starvation for 24 hours, full-thickness fetal membrane explants and primary chorion cells were treated with H2O2 at 100, 300, and 500 ¦ÌM for 24 hours. Cells were fixed for cell senescence-associated ¦Â-galactosidase assay. Cell lysates were harvested for quantitive reverse transcription polymerase chain reaction to quantify SIRT3 messenger RNA. Cell lysates were harvested for Western blot to semi-quantify SIRT3 protein and p38 MAPK phosphorylation levels, respectively. To examine the role of PGRMC1, primary chorion cells underwent the same treatment mentioned above following PGRMC1 knockdown using validated PGRMC1-specific small-interfering RNA. Hydrogen peroxide significantly induced cell senescence and p38 MAPK phosphorylation, and it significantly decreased SIRT3 expression in full-thickness fetal membrane explants and chorion cells. These effects were enhanced by PGRMC1 knockdown. This study further demonstrated that oxidative stress¨Cinduced cell aging is one of the mechanisms of PPROM and PGRMC1 acts as a protective element for maintaining fetal membrane integrity by inhibiting oxidative stress¨Cinduced chorion cell aging
%K fetal membranes and chorion cells
%K oxidative stress
%K PGRMC1
%K PPROM
%K SIRT3
%U https://journals.sagepub.com/doi/full/10.1177/1933719118776790