%0 Journal Article
%T Endosomal Wnt signaling proteins control microtubule nucleation in dendrites
%A Alexis T. Weiner
%A Christin Folker
%A Christopher Kozlowski
%A Derek M. R. Nye
%A Dylan J. Barbera
%A Dylan Y. Seebold
%A Gregory O. Kothe
%A Jeffrey D. Axelrod
%A Jessica G. Stoltz
%A Kana Behari
%A Madeleine K. Zalenski
%A Matthew Keegan
%A Matthew Shorey
%A Melissa M. Rolls
%A Mit A. Patel
%A Pankajam Thyagarajan
%A Pedro Torres-Gutierrez
%A Rachel D. Swope
%A Song Song
%J -
%D 2020
%R 10.1371/journal.pbio.3000647
%X Dendrite microtubules are polarized with minus-end-out orientation in Drosophila neurons. Nucleation sites concentrate at dendrite branch points, but how they localize is not known. Using Drosophila, we found that canonical Wnt signaling proteins regulate localization of the core nucleation protein ¦ĂTubulin (¦ĂTub). Reduction of frizzleds (fz), arrow (low-density lipoprotein receptor-related protein [LRP] 5/6), dishevelled (dsh), casein kinase I¦Ă, G proteins, and Axin reduced ¦ĂTub-green fluorescent protein (GFP) at branch points, and two functional readouts of dendritic nucleation confirmed a role for Wnt signaling proteins. Both dsh and Axin localized to branch points, with dsh upstream of Axin. Moreover, tethering Axin to mitochondria was sufficient to recruit ectopic ¦ĂTub-GFP and increase microtubule dynamics in dendrites. At dendrite branch points, Axin and dsh colocalized with early endosomal marker Rab5, and new microtubule growth initiated at puncta marked with fz, dsh, Axin, and Rab5. We propose that in dendrites, canonical Wnt signaling proteins are housed on early endosomes and recruit nucleation sites to branch points
%K Neuronal dendrites
%K Microtubules
%K Green fluorescent protein
%K RNA interference
%K Wnt signaling cascade
%K Endosomes
%K Nucleation
%K Neurons
%U https://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.3000647