%0 Journal Article
%T Selective suppression and recall of long-term memories in Drosophila
%A Benjamin Escribano
%A Dominique Siegenthaler
%A Jan Pielage
%A Vanessa Br£¿uler
%J -
%D 2019
%R 10.1371/journal.pbio.3000400
%X Adaptive decision-making depends on the formation of novel memories. In Drosophila, the mushroom body (MB) is the site of associative olfactory long-term memory (LTM) storage. However, due to the sparse and stochastic representation of olfactory information in Kenyon cells (KCs), genetic access to individual LTMs remains elusive. Here, we develop a cAMP response element (CRE)-activity¨Cdependent memory engram label (CAMEL) tool that genetically tags KCs responding to the conditioned stimulus (CS). CAMEL activity depends on protein-synthesis¨Cdependent aversive LTM conditioning and reflects the time course of CRE binding protein 2 (CREB2) activity during natural memory formation. We demonstrate that inhibition of LTM-induced CAMEL neurons reduces memory expression and that artificial optogenetic reactivation is sufficient to evoke aversive behavior phenocopying memory recall. Together, our data are consistent with CAMEL neurons marking a subset of engram KCs encoding individual memories. This study provides new insights into memory circuitry organization and an entry point towards cellular and molecular understanding of LTM storage
%K Camels
%K Neurons
%K Memory recall
%K Memory
%K Green fluorescent protein
%K Long term memory
%K Conditioned response
%K Drosophila melanogaster
%U https://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.3000400