%0 Journal Article
%T Global Transcriptome Analysis and Identification of Differentially Expressed Genes after Infection of Fragaria vesca with Powdery Mildew (Podosphaera aphanis)
%A Jim M. Dunwell
%A Shridhar Jambagi
%J Open Journal of Immunology
%D 2015
%R 10.4172/2329-8936.1000106
%X Background: Podosphaera aphanis, the causal agent of strawberry powdery mildew causes significant economic loss worldwide.
Methods: We used the diploid strawberry species Fragaria vesca as a model to study plant pathogen interactions. RNA-seq was employed to generate a transcriptome dataset from two accessions, F. vesca ssp. vesca Hawaii 4 (HW) and F. vesca f. semperflorens Yellow Wonder 5AF7 (YW) at 1 d (1 DAI) and 8 d (8 DAI) after infection.
Results: Of the total reads identified about 999 million (92%) mapped to the F. vesca genome. These transcripts were derived from a total of 23,470 and 23,464 genes in HW and YW, respectively from the three time points (control, 1 and 8 DAI). Analysis identified 1,567, 1,846 and 1,145 up-regulated genes between control and 1 DAI, control and 8 DAI, and 1 and 8 DAI, respectively in HW. Similarly, 1,336, 1,619 and 968 genes were up-regulated in YW. Also 646, 1,098 and 624 down-regulated genes were identified in HW, while 571, 754 and 627 genes were down-regulated in YW between all three time points, respectively.
Conclusion: Investigation of differentially expressed genes (log2 fold changes ?5) between control and 1 DAI in both HW and YW identified a large number of genes related to secondary metabolism, signal transduction; transcriptional regulation and disease resistance were highly expressed. These included flavonoid 3กไ-monooxygenase-monooxygenase, peroxidase 15, glucan endo-1,3-?-glucosidase 2, receptor-like kinases, transcription factors, germin-like proteins, F-box proteins, NB-ARC and NBS-LRR proteins. This is the first application of RNA-seq to any pathogen interaction in strawberry
%K Shridhar Jambagi
%K Jim M. Dunwell
%K Disease resistance
%K Fragaria vesca
%K Gene expression
%K Podosphaera aphanis
%K Powdery mildew
%K RNA-seq
%K Transcriptome
%U https://www.longdom.org/abstract/global-transcriptome-analysis-and-identification-of-differentially-expressed-genes-after-infection-of-fragaria-vesca-wit-11848.html