%0 Journal Article
%T Characterization of Bone Marrow-Derived Dendritic Cells Developed in Serum-Free Media and their Ability to Prevent Type 1 Diabetes in Nonobese Diabetic Mice
%A Anna V Chernatynskaya
%A Ben M Looney
%A Chang-Qing Xia
%A Michael J Clare-Salzler
%J Open Journal of Immunology
%D 2014
%R 10.4172/2155-9864.1000206
%X Dendritic cells (DC) have been investigated as a cell-based therapy for Type 1 Diabetes (T1D). BM-DC expanded ex vivo with GM-CSF and IL-4 is typically cultured with fetal bovine serum (FBS). The effect of FBS on NOD BM-DC has not been extensively studied. In the present study we compare BM-DC generated in serumfree culture media (X-VIVO20; FBS-) with BM-DC generated in media containing 10% FBS (RPMI1640/10%FBS; FBS+). We show that FBS- BM-DC display a phenotype and cytokine-producing profile distinct from FBS+ BMDC. Additionally, compared to FBS+ BM-DC, we show evidence of an altered Th cell response induced by FBSBM- DC. Finally, we demonstrate that only FBS- BM-DC prevent the onset of T1D and induce increased levels of CD4+Foxp3+ regulatory T cells as well as a long-lasting ¦Â cell-specific T cell response. This study indicates that serum-free media generates a more tolerogenic BM-DC capable of preventing T1D in the NOD mice.
%K Ben M Looney
%K Anna V Chernatynskaya
%K Michael J Clare-Salzler and Chang-Qing Xia
%K Bone marrow-derived dendritic cells
%K Type 1 diabetes
%K Serum-free media
%K Fetal bovine serum
%K NOD mouse
%U https://www.longdom.org/abstract/characterization-of-bone-marrowderived-dendritic-cells-developed-in-serumfree-media-and-their-ability-to-prevent-type-1--28127.html