%0 Journal Article
%T First Description of Extended-Spectrum ¦Â-Lactamases and OXA-48 Carbapenemase in Enterobacteriaceae Isolates in Brazzaville, Congo
%A Christian Aim¨¦ Kayath
%A Edgarthe Priscilla Ngaiganam
%A Esther Nina Ontsira Ngoyi
%A Etienne Nguimbi
%A Fils L
%A Gabriel Ahombo
%A Linda Hadjadj
%A Seydina M. Diene
%A Simon Charles Kobawila
%A et Jean-Marc Rolain
%A ry Mpelle
%J Open Journal of Immunology
%D 2020
%R 10.35248.2327-5073.20.9.336
%X Objective: To characterize genotypically Extended-Spetrum-Beta-Lactamase (ESBL) and OXA-48 carbapenemases producing Enterobacteriaceae especially Klebsiella, Enterobacter, Serratia (KES) and Citrobacter species in portage and infection processes at the Brazzaville hospital center.

Material and Methods: The study was carried out for 7 months. Clinical samples (urine, pus, and blood cultures) were collected from inpatients and outpatients at the Brazzaville University Hospital. Strains were identified by API20E and confirmed by MALDI-TOF. Antibiotics susceptibility testing was performed on isolated strains by diffusion method on MH agar plates. ESBL and OXA-48 phenotypes were identified according to the CA-SFM synergy technique and by a decrease in inhibition diameter around the Ertapenem disk and confirmed by PCR and sequencing. MLST K. pneumoniae genotyping of OXA-48 strains was performed.

Thirty-four no duplicate Enterobacteria strains were isolated from thirty-four patients, of which 12/34 (35.29%) were from outpatients and 22/34 (64.70%) from internal patients. Except for imipenem, colistin; the amikacin and fosfomycin, tested antibiotics show high resistance to the majority of the beta-lactam, as well as a resistance very frequent aminoglycosides, to sulfamides, tetracyclines and Fluoroquinolones. PCR revealed that 30/34 (88.24%) produced ESBLs, of which 2 strains harbor both ESBL and OXA-48 enzymes. blaSHV gene was the most common ESBL gene detected with 20/30 (66.67%), blaCTX-M was detected in 14 isolates (60.87%), blaTEM 15/30 (50%), blaOXA-48 2/30 (6.67%), blaCTX-M-9 1/30 (3.33%). 70% of the isolates (n=24) were isolated from urine samples. Sequencing of the amplification products revealed that the blaCTX-M1 strains were all CTX-M15; 13 variant enzymes were detected for blaSHV. Four types for TEM. Both strains OXA-48 were OXA-181 non-plasmid-borne and CTX-M-9 was CTX-M-27. These strains were resistant to gentamycin and fluoroquinolone. MLST K. pneumoniae OXA-48 showed two different standard sequences known in the ST464 and ST15 literature.

Conclusion: We report here for the first time in Congo Brazzaville, the presence of ¦Â-lactamase genes including blaTEM, blaSHV, blaCTX-M and blaOXA-48 genes at disturbing frequencies within Enterobacteriaceae strains at the Brazzaville University Hospital. It proves the need to promote an infection prevention program with antibiotic regulation in hospitals in Congo Brazzaville
%K Simon Charles Kobawila
%K Gabriel Ahombo
%K Esther Nina Ontsira Ngoyi
%K Etienne Nguimbi
%K Christian Aim¨¦ Kayath
%K Edgarthe Priscilla Ngaiganam
%K Linda Hadjadj
%K Seydina M. Diene
%K et Jean-Marc Rolain
%K Fils Landry Mpelle
%K Enterobacteriaceae
%K Antibiotic resistance ESBL
%K OXA-48
%K MLST
%K CHU
%K Brazzaville
%U https://www.longdom.org/abstract/first-description-of-extendedspectrum-lactamases-and-oxa48-carbapenemase-in-enterobacteriaceae-isolates-in-brazzaville-c-53011.html