%0 Journal Article
%T Novel Cancer Vaccination System Based on Human Endo-and#914;-N-Acetyl Glucosaminidase Gene Delivery
%A Kagemasa Kajiwara
%A Masahiro Sato
%A Minoiru Kimura
%A Satoshi Watanabe
%A Seiki Haraguchi
%A Shin-ichiro Mugikura
%A Shingo Nakamura
%A Takayuki Sakurai
%J Open Journal of Immunology
%D 2014
%R 10.4172/2168-958X.1000106
%X Cancer vaccination elicits an immune response against specific glycans or proteins expressed on the cell surface after gene transfer has occurred. We previously demonstrated that N-acetylglucosamine (GlcNAc) residues exposed after digestion with endo-¦Â-galactosidase, a carbohydrate-digesting enzyme, elicited this type of immune response, probably as a result of the presence of natural antibodies recognizing GlcNAc residues in host animals. Treatment of a cell with endo-¦Â-N-acetylglucosaminidase (ENGase), an enzyme that cleaves the amide bond between the proximal GlcNAc residues at the side chain of an asparagine residue on N-glycans, also causes exposure of GlcNAc residues on the cell surface. In this study, we examined whether mouse melanoma B16 cells transfected with a human ENGase (hENGase) cDNA expression construct, are susceptible to an immune attack after subcutaneous grafting to the syngenic host. The recombinant B16 cells overexpressing hENGase had approximately 3-fold more cell-surface GlcNAc residues than their parental cells. The grafting experiment revealed that the tumor size was approximately one-tenth of that derived from wild-type grafted cells. Direct injection and subsequent in vivo electroporation of a hENGase expression vector into B16 solid tumors resulted in regression of the tumors. Our present results strongly suggest that the ENGase is a useful tool for novel cancer vaccination
%K Satoshi Watanabe
%K Seiki Haraguchi
%K Shingo Nakamura
%K Takayuki Sakurai
%K Shin-ichiro Mugikura
%K Kagemasa Kajiwara
%K Minoiru Kimura and Masahiro Sato
%K N-Glycan
%K Endo-¦Â-N-acetylglucosaminidase
%K Gene therapy
%K In vivo gene transfer
%K N-acetylglucosamin
%K B16 Melanoma
%K Immune response
%U https://www.longdom.org/abstract/novel-cancer-vaccination-system-based-on-human-endo914nacetyl-glucosaminidase-gene-delivery-10951.html