%0 Journal Article
%T Up-Regulation of 14-3-3and#963; in Hacat upon Co-Cultivation with HEPM
%A Cuong Hoang-Vu
%A Gabriel Magnucki
%A Gunter Klohs
%A Hanna Prenzel
%A Joanna Biaˋek
%A Johannes Wohlrab
%A Kathrin Hammje
%A Rainer Finke
%J Open Journal of Immunology
%D 2013
%R 10.4172/2155-9554.1000171
%X Background: To generate an in vitro system of the dermal epithelial-mesenchymal interaction we coˋ⊿ˋ?ˋ＆cultured HaCaT (keratinocytes) and HEPM (embryonic mesenchyme). This model allowed a local disjunction with preserved cell-cell communication.
Methods: For the analysis of different protein expression patterns between co- and pure-cultured HaCaTs we performed 2D-electrophoresis and mass spectrometry. Afterwards the mass spectromertically identified protein 14-3- 3考 was silenced by siRNA in HaCaT cells.
Results: We analyzed 28 spots and found 17 different expressed mainly metabolic and cytoskeletal proteins. Interestingly, stratifin (14-3-3考), maspin and Profilin-1 (PFN1) were up-regulated, whereas Peroxiredoxin-5 (PRDX5), PDZ-and-LIM-domain-protein-1 (CLIM1) and Annexin A1 (ANXA1) were decreased in co-cultured HaCaTs. The specific knock-down of 14-3-3考 resulted in a down-regulation of RhoA, Rac1/2/3, LIMK1 and phosphorylated cofilin, which are involved in cytoskeleton dynamics. Furthermore, reduction of 14-3-3考 coincided with significantly decreased proliferation rates and levels of Ki67.
Conclusions: We concluded that the interaction with mesenchymal cells may initiate the alternation of epidermal precursor cells to differentiated keratinocytes, what was confirmed by the up-regulation of different keratins. Furthermore, 14-3-3考 may influence the proliferation-rate of keratinocytes via Rho GTPases
%K Gabriel Magnucki
%K Joanna Biaˋek
%K Kathrin Hammje
%K Hanna Prenzel
%K Gunter Klohs
%K Johannes Wohlrab
%K Cuong Hoang-Vu and Rainer Finke
%K 14-3-3考
%K HaCaT
%K Keratinocytes differentiation
%K Small GTPases
%K Proliferation
%U https://www.longdom.org/abstract/upregulation-of-1433963-in-hacat-upon-cocultivation-with-hepm-7375.html