%0 Journal Article
%T Cytotoxic Effects of Drug-loaded Hyaluronan-Glutaraldehyde Cross-Linked Nanoparticles and the Release Kinetics Modeling
%A A Abdah
%A Gul-e-Saba
%A MA Abdullah
%J Open Journal of Immunology
%D 2014
%R 10.4172/2090-4568.1000104
%X In this study, paclitaxel (PTX)-loaded hyaluronic acid (HA) nanoparticles (NPs) were synthesized. FESEM and TEM showed HA-PTX NPs with spherical shape and sizes >100 nm. TheHA entrapment maintained the crystalline nature of PTX as suggested by XRD, and the entrapment was confirmed by the characteristic peak of PTX at 1736 cm-1 by FTIR. HA-PTX showed cytotoxicity towards lung (A549) (IC50 0.3 ¦Ìg/ml), breast (MCF-7) (IC500.2 ¦Ìg/ml) and colorectal (HT-29) (IC50 0.2 ¦Ìg/ml) cancer cells, with 2-3 fold more cytotoxic effects than free PTX. At 10 ng/ml in A549 cell lines, PTX induced apoptosis, whilst HA-PTX showed enhanced apoptosis. The release kinetics profile showed PTX release in a biphasic manner, with initial burst release of 60-70%, followed by a slow and uniform release. PTX release was best modeled by first-order kinetics, Higuchi and Korsmeyer-Peppas models suggesting that it was concentration dependent, with anomalous, non-Fickian diffusion as a predominant mechanism for the drug transport
%K Gul-e-Saba
%K A Abdah
%K MA Abdullah
%K Hyaluronan
%K Paclitaxel
%K Drug delivery
%K Drug release models
%K Cytotoxicity
%K Cisplatin
%U https://www.longdom.org/abstract/cytotoxic-effects-of-drugloaded-hyaluronanglutaraldehyde-crosslinked-nanoparticles-and-the-release-kinetics-modeling-9618.html