%0 Journal Article
%T Validation of Biomarkers in Gene Expression Datasets of Inflammatory
Bowel Disease: IL13RA2, PTGS2 and WNT5A as Predictors of
Responsiveness to Infliximab Therapy
%A Andr¨¢s Gyorffy
%A Andr¨¢s Szab¨®
%A Bal¨¢zs Gyorffy
%A Barna V¨¢s¨¢rhelyi
%A Jan Budczies
%A Luca Bartha
%A M¨¢t¨¦ Kormos
%J Open Journal of Immunology
%D 2014
%R 10.4172/jpb.1000329
%X Background: Some patients with inflammatory bowel disease (IBD) do not respond to infliximab (IFX) therapy. Gene expression studies revealed genes that may help to predict non-responding IBD patients. Our purpose was to validate the discriminating power of published genes.
Methods: Microarray datasets of IBD patients responding or non-responding to IFX were downloaded from GEO database (¡®transcriptomic arm¡¯). Published genes discriminating responding and non-responding patients were identified in PubMed (¡®biomarker arm¡¯). Using ROC-analysis, the discriminating performance of genes in ¡®biomarker arm¡¯ were re-tested in each datasets of ¡®transcriptomic arm¡¯. We also performed an independent analysis of colon biopsy datasets to identify novel discriminating genes.
Results: The transcriptomic arm of four GEO datasets (3 and 1 from colon biopsies and blood cells, respectively) included 99 patients (of those, 59 and 40 were IFX responder and non-responder, respectively). Of the 65 candidate genes reported in biopsy specimens 25 genes discriminated significantly (p<0.05) infliximab responders and nonresponders in the three biopsy datasets consistently. Of the 39 candidate genes reported in peripheral blood 9 genes provided significant discrimination after re-testing. Independent analysis of three biopsy datasets identified the top five genes. Three genes (IL13RA2, PTGS2 and WNT5A) were highly effective discriminator in each analysis.
Conclusion: This analysis identified IL13RA2, PTGS2 and WNT5A, three genes in colonic tissues of IBD patients as suitable to discrimination between patients responding and non-responding to IFX therapy. These genes encode proteins implicated in intestinal pathology; the high expression in non-responding patients may indicate important targets in IBD therapy.
%K Andr¨¢s Gyorffy
%K M¨¢t¨¦ Kormos
%K Luca Bartha
%K Andr¨¢s Szab¨®
%K Bal¨¢zs Gyorffy
%K Jan Budczies and Barna V¨¢s¨¢rhelyi
%K Infliximab
%K Gene expression
%K Inflammatory bowel disease
%U https://www.longdom.org/abstract/validation-of-biomarkers-in-gene-expression-datasets-of-inflammatorybowel-disease-il13ra2-ptgs2-and-wnt5a-as-predictors--33886.html