%0 Journal Article
%T A High-Yielding Enzymatic Process for Production of Bioactive and#946;-1,3-Glucan Oligosaccharides with Defined Size
%A Juping Yu
%A Ningzi Guan
%A Rachel Chen
%A Wei Liu
%J Open Journal of Immunology
%D 2018
%R 10.4172/2168-958X.1000131
%X The study is to develop a bioprocess to depolymerize curdlan for production of ¦Â-1,3 oligosaccharides with a uniform size. A codon-optimized Streptomyces laminarinase was expressed in an E. coli system, resulting in an enzyme preparation (19.1 U/L) highly active on solid curdlan substrate without any pre-treatment. In a preparative scale reaction at pH 5.5 and 55¡ãC, 90% conversion and a product concentration close to 27 g/L were achieved within 6 hours. The major product was identified as ¦Â3-glucan pentasaccharide based on HPLC, TLC, and MS analyses. A single-enzyme based bioprocess was highly efficient in deriving ¦Â3-glucan pentasaccharide from solid curdlan. Enzyme to curdlan ratio and reaction time are critical parameters for high-yield production of ¦Â3-glucan pentasaccharide from curdlan
%K Ningzi Guan
%K Juping Yu
%K Wei Liu and Rachel Chen
%K ¦Â-Glucan
%K ¦Â-Glucan oligosaccharide
%K Curdlan
%K Laminarinase
%K LPHase
%U https://www.longdom.org/abstract/a-highyielding-enzymatic-process-for-production-of-bioactive-94613glucan-oligosaccharides-with-defined-size-17312.html