%0 Journal Article
%T Development, Validation and Statistical Correlation of RP¨CLC Methods for Determination of Atazanavir Sulfate in Capsule Dosage Form
%A A. Behera
%A D. G. Sankar
%A S. K. Moitra
%A S. C. Si
%J Journal of Chemistry
%D 2012
%I Hindawi Publishing Corporation
%R 10.1155/2012/402970
%X To study the effective therapeutic bioavailability of Atazanavir Sulfate (ATV), administered singly or in combination with Ritonavir, a cost effective and rapid method is required. In order to assess an in-depth study, it is primarily thought prudent to develop an effective analytical method for estimation of ATV in marketed dosage forms. The present work is to develop a simple and precise analytical method for in depth evaluation of therapeutic efficacy of ATV. The novelty of the method shows linearity in the concentration range of 10-100 µg/mL at two wavelengths, i.e. 254 nm and 284 nm respectively. The chromatographic system consists of HiQSil C18HS column; an isocratic mobile phase consisted of methanol and tetrahydrofuran (95:5 v/v). The developed method is validated according to ICH guidelines in capsule dosage form. Validation of the developed method shows good result in range, linearity, accuracy and precision. Student¡¯s t¨Ctest was used to correlate the two methods and applied to raw materials and capsule dosage form.
%U http://www.hindawi.com/journals/chem/2012/402970/