%0 Journal Article
%T Proteomic Analysis of Sera from Common Variable Immunodeficiency Patients Undergoing Replacement Intravenous Immunoglobulin Therapy
%A Giuseppe Spadaro
%A Concetta D'Orio
%A Arturo Genovese
%A Antonella Galeotafiore
%A Chiara D'Ambrosio
%A Stefano Di Giovanni
%A Monica Vitale
%A Mario Capasso
%A Vincenzo Lamberti
%A Andrea Scaloni
%A Gianni Marone
%A Nicola Zambrano
%J Journal of Biomedicine and Biotechnology
%D 2011
%I Hindawi Publishing Corporation
%R 10.1155/2011/706746
%X Common variable immunodeficiency is the most common form of symptomatic primary antibody failure in adults and children. Replacement immunoglobulin is the standard treatment of these patients. By using a differential proteomic approach based on 2D-DIGE, we examined serum samples from normal donors and from matched, naive, and immunoglobulin-treated patients. The results highlighted regulated expression of serum proteins in naive patients. Among the identified proteins, clusterin/ApoJ serum levels were lower in naive patients, compared to normal subjects. This finding was validated in a wider collection of samples from newly enrolled patients. The establishment of a cellular system, based on a human hepatocyte cell line HuH7, allowed to ascertain a potential role in the regulation of CLU gene expression by immunoglobulins.
%U http://www.hindawi.com/journals/biomed/2011/706746/