%0 Journal Article
%T Feasibility of Treating Irradiated Bone with Intramedullary Delivered Autologous Mesenchymal Stem Cells
%A B¨¦reng¨¨re Phulpin
%A Gilles Dolivet
%A Pierre-Yves Marie
%A Sylvain Poussier
%A Sandrine Huger
%A Pierre Bravetti
%A Pierre Graff
%A Jean-Louis Merlin
%A Nguyen Tran
%J Journal of Biomedicine and Biotechnology
%D 2011
%I Hindawi Publishing Corporation
%R 10.1155/2011/560257
%X Background. We aimed to explore (i) the short-term retention of intramedullary implanted mesenchymal stem cells BMSCs and (ii) their impact on the bone blood flow and metabolism in a rat model of hindlimb irradiation. Methods. Three months after 30 Gy irradiation, fourteen animals were referred into 2 groups: a sham-operated group (=6) and a treated group (=8) in which 111In-labelled BMSCs (2¡Á106 cells) were injected in irradiated tibias. Bone blood flow and metabolism were assessed by serial 99mTc-HDP scintigraphy and 1-wk cell retention by recordings of 99mTc/111In activities. Results. The amount of intramedullary implanted BMSCs was of 70% at 2 H, 40% at 48 H, and 38% at 168 H. Bone blood flow and bone metabolism were significantly increased during the first week after cell transplantation, but these effects were found to reduce at 2-mo followup. Conclusion. Short-term cell retention produced concomitant enhancement in irradiated bone blood flow and metabolism.
%U http://www.hindawi.com/journals/biomed/2011/560257/