%0 Journal Article %T Explanting Is an Ex Vivo Model of Renal Epithelial-Mesenchymal Transition %A Catherine E. Winbanks %A Ian A. Darby %A Kristen J. Kelynack %A Dodie Pouniotis %A Gavin J. Becker %A Tim D. Hewitson %J Journal of Biomedicine and Biotechnology %D 2011 %I Hindawi Publishing Corporation %R 10.1155/2011/212819 %X Recognised by their de novo expression of alpha-smooth muscle actin (SMA), recruitment of myofibroblasts is key to the pathogenesis of fibrosis in chronic kidney disease. Increasingly, we realise that epithelial-mesenchymal transition (EMT) may be an important source of these cells. In this study we describe a novel model of renal EMT. Rat kidney explants were finely diced on gelatin-coated Petri dishes and cultured in serum-supplemented media. Morphology and immunocytochemistry were used to identify mesenchymal (vimentin %U http://www.hindawi.com/journals/biomed/2011/212819/